FastQCFastQC Report
Mon 27 Feb 2023
SRR3126737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781378
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.4665489300979354No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50980.28618294376600584No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45820.2572166042243701No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG38550.2164055018081508No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG30330.17026144928252174No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28670.1609428206702901No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG26590.14926646674652994No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC24820.13933033864794558TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42050.083.5031
CGAAGGG14900.083.269932
AGGGATG31050.081.431275
GAGGGTA10250.078.863324
TAGCGGG8300.078.705882
CGTAGGG4150.078.139652
ACGGGAT6050.077.681064
AGAGGGT15600.077.1233443
CTAACGG551.8189894E-1277.020991
AGTACGG2200.077.020991
AGGGTAC7100.076.78395
CGCTACG7900.076.7420043
TAAGGGA19200.076.125413
AAGGGAC14450.075.780694
AGTAAGG11500.075.7187041
ATAGCGG2750.075.309411
AAGGGCA25700.074.975824
AGGGCAT19000.074.947915
AGGGCAC12100.074.5738145
AAGGGTA13150.074.3375244
Produced by FastQC (version 0.11.5)