FastQCFastQC Report
Mon 27 Feb 2023
SRR3126736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536543
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190500.7510221588989424No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52740.2079207803691875No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37940.14957365201378411No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG31990.12611652946549692No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG26890.10601042442410792No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG26850.10585272948260684No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26370.10396039018459376No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC25770.1015949660620774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72000.083.8733751
CGTAGGG7700.082.052982
CGAAGGG23450.074.796352
ACGGGTA2900.074.787934
ACGGGAT7150.073.8556444
ATAGCGG4700.073.2776641
ATAGGGC15050.071.428263
ACGAGGG25150.071.2403262
AGGGCAT22250.070.753175
CGAGGGA14250.070.144353
AGGGATG32100.069.976785
AGGGCGA13300.069.605845
TAAGGGA23600.069.524673
GGTAAGG14700.069.323911
AACGAGG10900.069.253241
AGAGGGC31050.069.0913
AAGGGAC24500.069.087594
AGGGAAT34900.069.075355
AGTAAGG16750.068.72611
AAGGGCA37550.068.557414
Produced by FastQC (version 0.11.5)