FastQCFastQC Report
Mon 27 Feb 2023
SRR3126734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699617
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193280.7159534111690657No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52870.1958425954496508No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38080.14105704624026297No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG31860.11801674089324524No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC29710.11005264820898668No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG29390.10886729487923658No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG28960.10727447634238486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75450.085.1487051
CGTAGGG10100.078.1686252
ACGGGTA2450.074.807144
CGAAGGG24400.071.839542
GGTAAGG16350.070.814461
AGGGCAT21150.070.2149055
AGGGTAC12800.070.12555
TAGGGCA15450.069.959054
AGGGAAT33600.069.933185
AGGGATG33700.069.725675
ACGGGAT7150.069.669774
TAAGGGA27050.069.3186653
ATAGGGC17550.068.817763
AGACGGG17900.068.784852
ATAGCGG5550.068.6905061
AAGGGAC24450.068.617344
AGAGGGC32850.068.5243153
TAGCGGG15500.068.217432
AGGGCGA13100.068.160835
CGGGATA5800.068.061875
Produced by FastQC (version 0.11.5)