FastQCFastQC Report
Mon 27 Feb 2023
SRR3126733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618395
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233781.4445175621526265No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26840.16584332007946145No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23850.14736822592753934No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21460.13260050852851127No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT16730.10337402179319635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80500.088.4876561
ATAGCGG2950.076.757511
TACGGGT1250.075.419733
ACGGGTA1700.074.865184
AGGGATG26600.074.729715
TAGCGGG8200.073.582512
ATAGGGC9950.073.4300163
TAGAGGG29900.072.678882
AAGGGTA11050.070.386064
ATAGAGG11750.070.258971
AGAGGGC22550.069.8176043
TAGGGCG2850.069.4655464
CGTAGGG4150.069.2883452
AGTAGGG28050.068.7335742
ACGGGAT5300.068.4825444
GGTAAGG7100.068.435351
AGTAAGG10600.068.09061
GAGGGAT20600.067.5024954
AGGGTAC7600.067.398085
TATAGGG13200.067.137072
Produced by FastQC (version 0.11.5)