FastQCFastQC Report
Mon 27 Feb 2023
SRR3126732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618488
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225541.3935228435428624No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26840.16583379055019254No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24210.14958405623025936No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20740.12814429269787606No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT17640.1089906134614529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83850.087.446681
ATAGGGC8550.079.153693
AGGGATG27050.075.0575
ATAGCGG3250.073.852591
TAGCGGG8550.073.15922
GCATAGG4000.072.947531
ACGGGAT4900.072.894074
ATAGAGG13250.070.3279951
GTACGGG4050.069.675432
GTAGGGT5600.069.6573
TAGAGGG31850.069.549642
GGGCGAT6850.069.2955866
TAGGGCG3150.068.631264
CGTAGGG4250.068.609812
GGTAAGG9250.068.177641
AAGAGGG55950.067.919532
GAATAGG10350.067.7524261
AGGGAAT18650.067.535285
TAGGGCA8750.067.139284
AGTAGGG29750.066.870852
Produced by FastQC (version 0.11.5)