FastQCFastQC Report
Mon 27 Feb 2023
SRR3126731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706662
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232551.3626013821131542No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26930.1577934002163287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24540.14378945567429285No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21450.12568393741701636No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT17440.10218777941970936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86800.086.888691
AGGGATG27400.074.2703865
TACGGGT1350.073.1035463
TAGAGGG33250.071.6587452
CGTAGGG4900.070.9722442
ACGGGAT5650.070.7047964
AGTAGGG29850.069.744882
CGAAGGG15800.069.6003952
AGAGGGC24400.068.951883
TAGGGCA9850.068.707584
AATGCGG4400.068.434761
AGGGTAC7150.068.3605655
GAGGGAT21000.068.258844
GGTAAGG10150.067.676251
GGGCGAT7650.067.578676
ATAGAGG13200.066.65261
TAGCGGG9800.066.176832
AGAAGGG60550.066.049322
GAGGGTA10750.066.015644
ATAGGGC10650.065.749063
Produced by FastQC (version 0.11.5)