FastQCFastQC Report
Mon 27 Feb 2023
SRR3126730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783651
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255201.4307731725544963No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33770.18933076033371998No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27820.15597221653787652No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25650.1438061593888042No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT22840.12805195635244787No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT19100.10708372882363199No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG17850.1000756313875304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93600.088.626311
CGTAGGG5850.080.617922
AGGGATG32100.079.198795
ACGGGAT4850.077.7921454
AGGGCAT16200.073.679235
ATAGGGA17650.073.481073
TAGGGCG2700.073.362314
GTACGGG4050.073.362312
TAGGGCA10550.073.312644
GAATAGG11400.072.435741
TAGGGTA5700.071.983324
GTAGGGT6400.071.479133
AGAGGGC25150.071.070393
AGGGAAT25550.070.810265
ATAGGGC11400.070.742233
TAACGGG5450.070.093222
TAGAGGG29300.069.856942
CGCTACG7250.069.603843
GGTAAGG10450.069.538311
GTAGGGC8150.069.440223
Produced by FastQC (version 0.11.5)