FastQCFastQC Report
Mon 27 Feb 2023
SRR3126729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789213
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246711.378874398967591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33760.1886863106852007No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27390.15308406545224074No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26730.14939529279074096No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT22340.12485936554228032No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT19070.10658317371939506No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG18620.10406810145019067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91700.088.5179441
ATAGCGG3600.079.733151
TAGGGCA10750.079.1289144
AGGGATG30750.077.7924965
ACGGGAT5100.076.484414
GTAGGGC9700.074.1284263
TAGCGGG7950.073.339532
AGGGTAC7250.073.249645
GGTAAGG10700.072.5624161
AGGGAAT23850.072.5144355
CGTAGGG5800.072.151542
AAGGGTA12150.071.558384
GTAGGGT6450.071.4054643
AGGGCAT15150.071.347755
ACGGGTA1650.071.206744
TAGGGCG2200.070.4946754
ATAGGGC11750.070.3946843
GGATGGC23250.070.1409457
CGAGGGA10050.069.676343
AGTAGGG30600.069.608192
Produced by FastQC (version 0.11.5)