FastQCFastQC Report
Mon 27 Feb 2023
SRR3126728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916642
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258241.3473564703267487No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35000.18261104577693696No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28290.14760189957227277No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26610.13883656937497976No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT24140.1259494470015788No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT22220.1159319267761011No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG20190.10534048612103877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98500.088.187811
AGGGATG31700.077.9801565
TAGGGCG3500.076.535774
ATAGCGG4400.073.792041
CGTAGGG5100.072.797172
AGTAGGG31100.072.684582
AGGGAAT24850.071.297215
GTAGGGT6350.071.048543
TAACGGG6300.070.866452
TAGGGTA6300.070.866454
ACGGGAT4250.070.769914
ATAGGGC13350.070.405463
GTAGGGC10450.069.706463
AGGGCAT14500.069.3590855
AATGCGG3800.069.345381
AGAGGGC26650.068.9504553
TAGCGGG9150.068.824222
AAGGGAT34100.068.770754
TAGGGCA10100.068.39964
TGTAGGG14450.068.2981642
Produced by FastQC (version 0.11.5)