FastQCFastQC Report
Mon 27 Feb 2023
SRR3126727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313174
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157740.6819201668356984No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44420.19203051737569243No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43640.18865852720115306No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33110.14313665984487117No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27230.11771704160603569No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25370.10967614195905712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60450.081.79231
GGTAAGG13500.075.4861761
TAAGGGA19500.073.96713
AAGGGTA16900.073.632414
AGGGCAT18250.073.386735
AGTAGGG36450.073.32412
CGTAGGG8200.073.004742
ATAGCGG4400.072.912781
TAGGGCG4850.072.8905644
TAGAGGG42100.072.2168962
AGGGATG34100.072.212515
ATAGGGC15250.072.017463
CGAAGGG23450.071.559672
CGGGTAT2500.071.429755
AGTAAGG13750.071.025631
AGAGGGC30100.070.938743
AGGGTAC11250.070.17665
AGGGCAC15150.069.171595
AACGAGG8700.068.8702851
AGGGTAT14600.068.558655
Produced by FastQC (version 0.11.5)