FastQCFastQC Report
Mon 27 Feb 2023
SRR3126726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316475
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152240.6572054522496465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42830.18489299474416948No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42180.18208700719843726No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33120.14297585771484692No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26450.11418210859171801No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24690.10658435769865851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58250.081.696641
AGGGCAT18900.075.592025
AGTAAGG14850.075.439631
AGGGATG30500.072.57475
CGAAGGG21450.071.90892
ATAGGGC14600.071.78213
GGTAAGG13950.071.1962661
AGTAGGG34450.071.118832
AGAGGGC30650.070.3795243
AGTACGG4150.070.322221
AAGGGCA31850.069.793644
TAAGGGA20550.069.751283
CGTAGGG7150.069.716552
ATAGCGG4600.069.582511
AGGGTAC9600.068.536355
ACGGGTA2950.068.503164
AGGGTAT14050.068.2366945
AAGAGGG75900.068.215272
GTAAGGG36550.067.804632
TAAGAGG18200.067.760861
Produced by FastQC (version 0.11.5)