FastQCFastQC Report
Mon 27 Feb 2023
SRR3126725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448066
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158610.6478991987961109No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46700.1907628307406745No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42680.1743417048396571No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34710.1417853930408739No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26770.10935162695776993No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25390.10371452403652515No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG25290.10330603831759438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61100.079.950671
CGTAGGG6650.077.0312962
AGGGCAT21050.076.3548055
ATAGGGC17250.074.648973
AGTAGGG38750.074.466172
AGGGATG30350.072.933085
TAGGGCG5300.072.711044
TAAGGGA20050.072.4283
GGTAAGG14150.072.172091
AGTACGG4000.071.768821
ACGGGTA3150.071.61324
GTAGGGT8900.070.230223
AGTAAGG16100.070.1538241
TAGTAGG11050.069.846971
AGAGGGC30850.069.770643
ATAGCGG5600.069.75191
TAGGGCA14650.069.612064
AGCAGGG49250.069.468442
AAGAGGG84750.069.0386052
TAGCGGG12950.068.226092
Produced by FastQC (version 0.11.5)