FastQCFastQC Report
Mon 27 Feb 2023
SRR3126723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054140
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52160.494810935928814No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.3842943062591307No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG28990.2750109093668773No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG28980.274916045307075No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26860.25480486462898666No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC26640.252717855313336TruSeq Adapter, Index 14 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC22240.21097766900032255No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21380.20281935985732447No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA14330.13594019769670063No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGT13770.13062781034777166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT25650.085.69541
CGTAGGG1600.085.2383042
TAGCGGG5650.084.9001542
ACGGGTA1300.083.1481864
TTAGCGG1550.081.9898451
GTAGAGG6700.080.788771
TACGCGG353.8256985E-880.6884161
GCGTAGG700.080.6884161
TCGAAGG1750.080.688411
TAAGGGT3450.080.371333
AGGGCGA4950.079.7521445
CGAAGGG8150.079.630292
AGTAAGG5700.079.272831
AGGGCAT9150.079.0984345
AGGGAAC7150.078.218455
CAGGGTA2300.077.646884
GGTAAGG6150.077.2990651
AAGGGTC3650.077.2550054
AGCGGGT2500.077.074753
AGGGTGC2750.076.9038545
Produced by FastQC (version 0.11.5)