FastQCFastQC Report
Mon 27 Feb 2023
SRR3126719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067564
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149570.7234117057561459No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44040.21300428910544003No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG32930.1592695558638088No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31230.15104731945419828No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26500.12817015579687013No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG26470.12802505750728876No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG25490.12328518004763092No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC25170.12173746495876307TruSeq Adapter, Index 16 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21490.10393874143678263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59900.084.199771
ATAGCGG4300.078.7783661
CGAAGGG19000.075.4407042
AGGGCAT18500.075.193365
CGTAGGG5800.074.545142
TAGCGGG11350.073.288692
GGTAAGG13850.073.0350951
AGTAAGG12250.072.588631
GTAAGGG31900.071.009412
AGGGATG28050.070.703215
GAATAGG13000.070.572281
ACGGGAT4600.070.493784
AGAGGGC26550.070.272523
TAAGAGG13800.069.54951
TAAGGGA18550.069.417053
ATAGGGC13500.069.275373
TAGGGCA11950.069.215654
AGTAGGG33750.069.205742
GGGGGGA347250.068.778386
AAGGGAC18950.068.695784
Produced by FastQC (version 0.11.5)