FastQCFastQC Report
Mon 27 Feb 2023
SRR3126718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542347
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116780.7571577602186798No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38030.24657226940500418No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26480.17168639741899844No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24180.15677405927459903No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT22310.1446496800006743No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC22010.1427045924166222No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG20250.13129341192351657No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG18970.12299437156489428No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17000.11022162976295218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50150.085.408251
ATAGCGG2300.082.0383761
TAAGGGA13100.076.284913
AGGGCAT14100.075.322345
CGAGGGA8450.075.3097463
AGGGATG19600.075.045175
AGTAAGG9900.074.808231
AGTAGGG24800.074.508962
CGAAGGG14550.074.190212
GGTAAGG9000.072.854641
CGTAGGG4600.072.7569662
ACGGGAT3700.072.618494
GCGTAGG2150.072.403641
AAGGGAC14850.072.3739854
AGGGTAT7200.071.14245
AAGGGTA9250.070.8348854
GAGGGTA6800.070.014274
AGGGCGA8600.069.396855
TAGGGCA9850.069.391444
TAGAGGG28400.069.379642