FastQCFastQC Report
Mon 27 Feb 2023
SRR3126717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547443
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110300.712788774772318No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37450.24201214519694747No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26950.17415827271182202No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23930.15464220653038593No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC21960.1419115275974624No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT21290.1375818043055544No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG21040.1359662359130514No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG19260.12446338895843014No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16440.10623977749099645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46000.086.0159151
CGTAGGG4350.078.910622
ACGGGAT3250.078.083564
AGGGCAT14250.077.1702965
ATAGCGG2350.076.077521
GTAGGGT5400.074.843473
AGTACGG2350.074.0754851
CGAAGGG14000.073.22012
GACCGAT900.073.102928
AGTAGGG25500.073.022532
GGTAAGG9000.072.662931
TAGCGGG7200.072.492382
AGTAAGG8650.071.79571
GTAGGGC8400.071.611023
AGGGATG21500.071.257135
TAGGGTC4650.070.744764
TGTAGGG13000.070.533132
GGGCGAT7750.069.7341166
TAGAGGG27100.069.405292
ATAGGGC9150.069.3364943