FastQCFastQC Report
Mon 27 Feb 2023
SRR3126715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094504
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263431.2577202048790548No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31600.15087104154491948No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28820.13759820940900566No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28810.13755046540851676No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22240.10618265708731041No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC21270.10155148903988724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92850.087.394091
AGGGATG38400.078.934725
TAGGGCA11900.075.639934
TAAGGGA19700.074.397593
AGGGTAC8100.073.681475
AGGGCAT20050.073.127355
ATAGGGC13900.072.554393
AAGGGTA14150.071.938614
CGTAGGG5700.071.929842
AGTAGGG35400.071.754682
GTAGGGC10600.070.245083
TACGCGG1950.070.1207351
ACGGGAT6150.069.731824
GAATAGG13050.069.370341
ATAGGGA21550.069.104213
CGAAGGG19450.069.0541842
ATAGCGG4550.068.393631
GAGGGAT29100.068.17954
TATAGGG16450.067.896472
AAGGGCG9500.067.465234
Produced by FastQC (version 0.11.5)