FastQCFastQC Report
Mon 27 Feb 2023
SRR3126714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088596
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250061.1972636163240762No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31060.14871234073032794No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29200.13980683674583308No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28210.13506681043150517No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21390.10241329582169073No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC21270.10183874717752978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90200.085.517671
AGGGATG38150.075.39225
GAATAGG13750.073.590061
CGAAGGG23000.072.7744452
AGTAGGG36050.072.514732
GAGGGAT28200.071.828644
AGGGCAT16950.071.535165
CGTAGGG5300.070.9693452
AGGGTAC7750.070.3437965
TAGGGCA11350.069.977814
TATAGGG16950.069.901672
ATAGCGG4650.069.836031
GGTAAGG13150.069.074061
AGTAAGG12100.068.8448261
TAAGGGA20250.068.6967543
AAGGGTA14250.068.599044
AAGGGAT39700.067.950234
AGGGAAT28050.067.8565445
GGGCGAT9850.067.751926
ACGGGAT6800.067.730884
Produced by FastQC (version 0.11.5)