FastQCFastQC Report
Mon 27 Feb 2023
SRR3126713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214717
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260071.1742809577928015No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32020.1445782914927731No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29840.13473504741237818No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29610.13369654000940076No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC23820.1075532449518381No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22340.10087067557615714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93050.087.1775361
AGGGATG42000.074.8584065
AGGGCAT20050.074.537795
CGTAGGG6350.074.008262
ATAGGGC13500.072.407493
TAGCGGG10700.072.03012
AGTAGGG34800.071.573222
ATAGCGG5250.070.802971
GGTAAGG13550.070.49211
TAAGGGA21800.070.0617143
GAGGGAT29350.069.333574
CGAAGGG20750.068.624392
GAATAGG16100.068.094771
TAGGGCA12750.067.822124
AAGGGAT42050.067.3930364
GGATGGC29500.067.228627
TAGAGGG38850.066.773172
GAAGGGC29800.065.76183
AAGGGAC22950.065.73344
TGAAGGG37900.065.718952
Produced by FastQC (version 0.11.5)