FastQCFastQC Report
Mon 27 Feb 2023
SRR3126712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741469
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38160.5146540179022994No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.5017067470116755No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27860.37574059063831394TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG26040.35119472290817283TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC24810.33460603207956097TruSeq Adapter, Index 16 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21780.29374120833102935No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT20040.27027427984177355No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGT19670.26528418585267893No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT10760.14511732789907603No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT10620.1432291842275267No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT10050.13554174213621878No Hit
GCAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT9760.13163058738800948No Hit
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9420.12704509561424687No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT9230.1244826149171442No Hit
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9170.12367341048648021No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA8530.11504189655939763No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA7830.10560117820165105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG800.094.293281
ATAGCGG1400.094.293281
CGAGGGT750.094.26143
CGAAGGG6000.089.548332
CAAGGGT850.088.7166063
AAGGGTA3850.088.1405264
AGGGCTC1600.088.113815
AACGAGG1950.087.039951
TAGCGGG3500.086.181852
GCGAAGG2850.086.021941
CGAGGGC1700.085.9442143
CGTTTTT22800.085.8151551
ATACAGG1100.085.721171
AGTAAGG4950.085.721161
GCATAGG1050.085.312971
ACGGGAT1050.085.284124
AGGGACA4300.085.244995
GTAGCGG1000.084.863951
GGGCGAT3500.084.589266
CGGGATA1000.084.589265
Produced by FastQC (version 0.11.5)