FastQCFastQC Report
Mon 27 Feb 2023
SRR3126711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747912
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36920.49364096310795924No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34410.4600808651285178No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG28330.3787878787878788TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC26210.3504422980243665TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG25310.3384087967568377TruSeq Adapter, Index 13 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCCACTT22290.2980297147257966No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21580.2885366192814128No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT19600.2620629164928494No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGT18070.24160596433805046No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT11270.1506861769833884No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT10720.14333237065323193No Hit
GCAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT9990.1335718640695697No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9890.13223480837317758No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT9190.12287541849843298No Hit
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9180.12274171292879375No Hit
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9010.12046871824492722No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG8670.11592272887719411No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA7930.10602851672389264No Hit
ACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG7930.10602851672389264No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA7680.10268587748291243No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA7640.10215105520435558No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA201.8359566E-594.0156594
GGTCGAC156.904033E-493.990517
ATCGGGT156.904033E-493.990513
AATGCGG1350.090.588111
GCGAAGG3500.088.696711
CGTAGGG1750.088.6670462
GTCGAGG800.088.192751
CGAAGGG6600.087.628932
AGTAAGG4850.087.283551
GTAGAGG4150.087.271861
AGCTAGG1350.087.103951
AAGGGTA3950.086.851994
ATTGCGG600.086.232911
AGGGCAT7150.086.103195
TAAGGGT2850.085.7457353
GAAGGGT3850.085.445923
TCGAAGG1050.085.113011
TCCGCTA500.084.665041
AAGTCGG500.084.665041
CGTTTTT22100.084.4947741
Produced by FastQC (version 0.11.5)