FastQCFastQC Report
Mon 27 Feb 2023
SRR3126710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777818
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39120.5029454191083261No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.4655330681470472No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30230.38865132974551886TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG28380.36486684545742065TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC27420.3525246265835967TruSeq Adapter, Index 16 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22300.2866994592565356No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGT21020.27024316742477034No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT20050.2577723837710107No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCCACTT17160.22061716236960316No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT11840.15222069944382877No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT11730.15080648686453643No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT11150.1433497296282678No Hit
GCAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT10840.13936422145026214No Hit
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT10730.1379500088709698No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT10350.1330645472334145No Hit
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT9870.12689343779650253No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA8040.10336608306827562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAGG800.094.1561051
TAGTCGG201.8224442E-594.1561051
GCGTAGG950.094.1561051
ATACCGG156.855999E-494.15611
ATCGGGT201.8388195E-593.986633
CGGGTAA254.923859E-793.9866265
TCGGGTA156.905272E-493.9866264
ACGGTCC156.905272E-493.9866265
AGGGTAT2950.092.393645
CGAAGGG6750.091.898032
TAGGGCG1100.089.714514
ATAGCGG1900.089.200521
GAGGGTA2300.087.857064
CGTTTTT23250.087.2715761
AAGGGTA4100.087.109564
ATTGCGG650.086.913321
CGAGGGT1250.086.46773
CGTAGGG1750.085.930632
AGGGCAT7500.085.841125
AGTAAGG3700.085.249451
Produced by FastQC (version 0.11.5)