FastQCFastQC Report
Mon 27 Feb 2023
SRR3126709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488774
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.5607970047838019No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49350.33148080232459726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44640.2998440327410339No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG35300.23710784847129246No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31970.21474045086762664No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG31940.21453894278110713No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC29050.1951269971130608Illumina Single End Adapter 1 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22910.15388500873873404No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT18150.12191239234430479No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA16820.11297886717527307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG4250.092.103612
CGTTTTT36350.085.4484941
CGCTACG10800.084.716013
GCTACGA10800.084.716014
TAGGGCG2750.084.033194
AGGGATG28200.082.652615
TCCGCTA11300.081.876741
GCGTAGG2200.081.534951
ACGGGAT5450.081.342744
CGAGGGA7550.080.580553
CTACGAC11400.079.968865
TAGCGGG7400.079.664672
AGGGCAT13250.079.088435
AGGGTAC5950.078.9781655
GGTAAGG8900.078.4972841
CGAAGGG13500.078.2531362
TCGAGGG4400.078.245192
ATAGCGG3000.077.10061
AGGGAAC13100.076.7655645
GTAGGGT5300.076.5261843