FastQCFastQC Report
Mon 27 Feb 2023
SRR3126707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544254
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84820.5492619737426615No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49510.3206078792737464No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45580.2951586979862121No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG38310.2480809504135978No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG35300.2285893382824328No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT33710.218293104631751No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC33120.21447248962929677Illumina Single End Adapter 1 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22850.1479678861119997No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT20930.13553469830740278No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA16930.10963222371449258No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGT15910.10302709269330046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG4400.088.657322
CGTTTTT38050.086.569971
ACGGGTA1850.086.376654
AGGGCAT13600.081.902785
CGCTACG8150.081.887933
TCCGCTA8450.080.748631
TAGCGGG6700.080.670032
CGGGATA2700.080.072475
AGGGATG28350.079.740915
ACGGGAT5850.079.5368654
ACGTAGG2900.079.510031
GCTACGA8400.078.891284
AAGGGCA18700.078.666884
GGTACGA301.4565521E-678.331777
CGAGGGA7250.077.791553
GGTAAGG8300.077.105381
TAGGGTC3550.076.78724
TAGGGCG2150.076.51014
AAGGGAC11300.076.1135254
CTACGAC8750.075.735635
Produced by FastQC (version 0.11.5)