FastQCFastQC Report
Mon 27 Feb 2023
SRR3126706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113713
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277801.3142749275800452No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54730.2589282461715474No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26950.1275007534135429No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23430.11084759378401893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95450.086.589951
ATAGCGG4450.074.207421
AGTAGGG33150.073.9609152
CGTAGGG6550.073.424552
AGTAAGG14000.073.120811
GAATAGG14600.073.023871
AGGGCAT17600.072.892275
ATAGGGC12600.072.2219543
AGGGATG26500.072.1738745
TAGGGCA11200.071.567094
AGAGGGC26800.071.252933
ACGGGTA1600.070.725134
GTAGGGC9250.069.3233643
GTAGGGT6750.069.153463
ATGTAGG7200.068.796461
TAGGGTC5900.068.727244
CGAAGGG21800.068.562282
AATAGGG35550.067.641472
GGTAAGG12500.067.55421
GCATAGG4900.067.392451
Produced by FastQC (version 0.11.5)