FastQCFastQC Report
Mon 27 Feb 2023
SRR3126705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117825
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264031.246703575602328No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53950.2547424834440995No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26180.1236173904831608No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24900.11757345389727669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96600.086.2716141
ATAGCGG4350.077.887851
ATAGGGC12750.074.8269963
TAGGGCA11700.073.91024
GAATAGG14050.073.014091
GTACGGG6200.072.054442
AGTAAGG15700.071.035451
AGTAGGG33400.070.678312
AGCAGGG41250.069.882012
AATAGGG33250.069.158582
ATAGAGG14050.068.9949651
AGGGATG24350.068.903565
AGGGACT34450.068.75655
GCATAGG5750.068.744491
TGTAGGG15100.067.578992
CGTAGGG6700.067.379112
AGAGGGC28650.067.2560963
TAAGGGA17850.066.612353
ATAACGG2050.066.568791
TAGCGGG11400.066.000012
Produced by FastQC (version 0.11.5)