FastQCFastQC Report
Mon 27 Feb 2023
SRR3126704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234011
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278181.2452042536943642No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55790.24973019380835634No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26610.11911311090231874No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23890.1069377008439081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98550.087.941141
GAATAGG14000.073.599551
AGTAAGG15200.073.051221
ATAGGGC13150.072.549333
CGTAGGG6500.072.301912
AGTAGGG33100.072.269142
ATAGCGG5500.070.147191
AGGGCAT18800.069.494445
AGGGATG25150.069.326465
AGGGAAT25450.069.063245
GTACGGG5800.068.87382
AGGGTAC8950.068.7877965
TGTAGGG16750.068.740772
TAGGGCA12950.068.58914
AATAGGG35400.068.502992
ATAGGGA19950.068.0797653
AGCAGGG43400.067.354062
AGAGGGC31750.067.200923
TAGAGGG41700.067.169692
TAAGGGA17800.066.5340043
Produced by FastQC (version 0.11.5)