FastQCFastQC Report
Mon 27 Feb 2023
SRR3126703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418407
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230230.9519902977455821No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59610.24648456608006838No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35070.14501281215279313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84900.086.312491
GGTAAGG14300.073.5832061
AGGGATG34800.073.459525
GAATAGG16200.072.817351
TAGGGCA13900.071.920314
CGTAGGG5450.071.814492
AGTAGGG38800.070.368342
TAGAGGG40750.069.199682
TAAGGGA21350.069.131653
ATAGGGC15150.068.78763
AGTAAGG16100.068.580381
AAGGGTA16300.068.5632254
AGGGCAT22450.068.447855
GTAAGGG40650.068.3258742
AATAGGG39250.067.87962
AGAGGGC33150.067.852423
ACGGGAT5750.067.247564
AAGGGCA37150.067.020184
TAGCGGG13200.066.803342
GTAGGGC12500.066.77193
Produced by FastQC (version 0.11.5)