FastQCFastQC Report
Mon 27 Feb 2023
SRR3126702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423522
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219560.9059542269473931No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57000.23519489404263713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33480.13814605355346476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81800.084.886221
CGTAGGG7350.077.4190752
AGGGATG36600.075.887385
AGTAGGG39850.072.694632
TAGGGCA14550.072.351614
ATAGAGG13700.072.1601261
GAATAGG16000.071.7907261
ATAGGGC14900.071.5983053
ACGGGAT6050.070.688564
CGAAGGG24050.070.394282
TAAGGGA19350.069.462153
ATAGCGG5300.069.2814941
GGTAAGG14100.069.111411
AATAGGG37650.068.323852
AGTAAGG16200.068.289091
AACGAGG8800.067.9390641
AGACGGG16350.067.592752
AAGAGGG81300.067.3884352
AGAGGGC32150.067.3880843
GTAGGGT9800.066.6579363
Produced by FastQC (version 0.11.5)