FastQCFastQC Report
Mon 27 Feb 2023
SRR3126701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582841
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230300.8916538029247639No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59180.22912753824180426No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35620.13791015397386056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85800.085.6152951
AGGGATG37850.074.003545
ACGGGTA3650.072.1053164
TAGGGCA14200.071.4887164
CGTAGGG6800.071.186962
AGTAGGG38450.070.893072
ATAGGGC15650.070.871193
AGTAAGG16400.068.578791
GTAGGGC12300.068.394323
TAAGGGA23900.068.03783
ATAGGGT10850.068.005183
GAATAGG17400.067.882881
TAAACGG3700.067.407761
AGGGCAT23600.067.309565
ATAGCGG5950.067.226071
TAGACGG3300.067.0223541
CGAAGGG24400.066.83622
AATAGGG38550.066.807972
GAGGGTA10850.066.272574
TATAGGG21650.065.991472
Produced by FastQC (version 0.11.5)