FastQCFastQC Report
Mon 27 Feb 2023
SRR3126700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151560
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284151.3206696536466564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50350.23401624867537973No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28180.13097473461116585No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT22850.10620201156370261No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT22350.10387811634349031Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95250.087.422861
AGTAAGG14100.074.615841
ATAGGGC12300.073.613763
AGGGTAC8550.072.549425
AGTAGGG33600.071.72072
TAAGGGA17300.071.692313
GTAGGGC10500.070.962793
CGTAGGG6050.070.93312
TGTAGGG15000.069.795052
GAATAGG12000.069.588471
ATAGCGG4350.069.4122241
GGTAAGG12200.068.447681
AGGGATG26800.068.2088855
CGAAGGG20900.067.9177252
TAGAGGG35900.067.651232
AGGGCAT19200.067.3065955
TAGCGGG11400.067.015162
TAGGGCG3600.066.808334
TATAGGG19450.066.6769942
ATAGGGA18550.066.607063
Produced by FastQC (version 0.11.5)