FastQCFastQC Report
Mon 27 Feb 2023
SRR3126698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246594
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284411.2659608278131251No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52610.234176713727536No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27780.12365385111862669No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT25130.11185821737260937No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT24300.10816373585970585Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96550.086.464141
CGTAGGG6550.076.7692262
TAGCGGG12550.075.6401442
ATAGGGC13150.075.405223
GAATAGG12950.073.040041
GTACGGG5950.070.2939152
AAGGGTA13900.069.984244
AGGGATG30300.069.328145
AGTAAGG13400.069.182491
GGTAAGG12500.068.893111
AGTAGGG34550.068.6891252
AGGGTAC9550.068.45
ATAGAGG15700.068.33921
ATAGCGG6000.068.234291
ATAGGGA19700.067.98663
AGGGAAT25350.067.8496865
GCATAGG7150.067.790041
TAAGAGG16000.067.6460651
AGGGCAT16750.067.3350455
TATAGGG18450.066.989092
Produced by FastQC (version 0.11.5)