FastQCFastQC Report
Mon 27 Feb 2023
SRR3126695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466261
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269141.0912875806737405No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54730.2219148743786647No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34920.14159085352280235No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC26840.10882870872142081TruSeq Adapter, Index 15 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26410.10708517873817897No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26010.10546329038167493No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG25150.10197623041519127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98500.087.5270541
CGTAGGG7000.075.192392
AGTAAGG15750.074.9974061
TAGGGCA11750.073.592564
TAGGGTC6400.072.695774
TAAGGGA19850.072.446073
ATAGCGG5800.072.212951
GGTAAGG14950.071.770621
ATAGGGC14800.071.763013
AAGGGAC21350.070.657964
CGAAGGG24150.070.638822
TAGGGTA6800.070.492874
TAGGGCG4750.069.256154
AGGGATG29350.069.011765
GTACGGG6200.068.976892
TGTAGGG16050.068.809242
GTAAAGG18950.068.541391
GGGCGAT11550.067.949846
AGGGCAT20350.067.894855
ATAGAGG18700.067.696111
Produced by FastQC (version 0.11.5)