FastQCFastQC Report
Mon 27 Feb 2023
SRR3126692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215812
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257761.163275584751775No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44320.20001696894862922No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27720.1251008659579423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97850.087.687321
ACGGGAT5700.073.378534
AGTAGGG34150.070.458462
ATAGGGC14650.070.252273
TAGGGCA12300.069.537674
AGGGATG26950.068.705475
ATAGCGG4800.068.638491
AGGGCAT15900.068.571675
TAGTAGG9300.068.322191
TAAGGGA17700.067.970523
ATGAGGG33450.067.85862
AGTACGG3700.067.419431
AGGGACT29050.067.297835
ATAAGGG31300.067.114612
CGTAGGG7800.066.877842
GTAGGGC9850.066.7952653
GGTAAGG11300.066.6426851
AGGGTAC8700.066.441545
AGAAGGG74950.066.401542
GTACAGG6350.065.967071
Produced by FastQC (version 0.11.5)