FastQCFastQC Report
Mon 27 Feb 2023
SRR3126691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394493
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239741.0012140357060972No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50130.20935538337343232No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35100.14658635460617342No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26080.10891658484698014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89700.085.4577941
ATAGCGG4850.076.885321
ACGGGTA2100.076.378624
CGTAGGG7200.074.6937942
GGTAAGG15400.073.8676761
AGGGTAC10250.071.519235
TAGGGCA13350.071.380954
ATAGGGC14950.071.3147663
CGAAGGG22800.071.176362
AGTAGGG39350.070.972342
ATAACGG2350.070.3004761
TAGAGGG45200.070.136332
AGGGCAT22850.069.510865
GAATAGG15000.068.914551
AGGGATG31450.068.5826345
TAGCGGG12900.067.654112
ATGAGGG39450.066.367912
ATAGGGA22200.066.087553
AACGAGG9650.066.033541
AGGGAAT28100.065.888865
Produced by FastQC (version 0.11.5)