FastQCFastQC Report
Mon 27 Feb 2023
SRR3126690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398055
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226300.9436814418351539No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49740.20741809508122208No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34560.14411679465233282No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25420.10600257291846933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85750.086.430731
CGTAGGG6450.075.8174442
ATAGCGG5100.072.89181
AGGGCAT23300.072.411515
ATAGGGC13050.072.025873
TAGGGCA11750.069.995354
AGTAGGG39200.069.932332
GGTAAGG14050.069.664061
TATAGGG16950.069.353092
TAGGGTC7000.068.481174
CGAAGGG24050.068.4303052
GAATAGG14800.068.3594361
ACGGGAT6600.068.35914
GTACGGG5800.068.099952
AGGGATG27200.068.076365
AGGGTAC9850.067.751855
GCATAGG6600.067.733171
AGACGGG16300.066.3492052
TAAGGGA19150.066.261923
TGTAGGG17400.066.208282
Produced by FastQC (version 0.11.5)