FastQCFastQC Report
Mon 27 Feb 2023
SRR3126689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514639
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237290.9436344540906269No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51370.20428379580528258No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36820.14642260777789573No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26880.10689407107739918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86550.086.592751
ACGGGTA2800.077.20564
GGTAAGG15400.075.153161
ATAGGGC13800.074.9191063
TAGGGCA13350.073.572244
AGGGCAT23400.072.9020545
AGTAAGG15250.070.647771
ATAGAGG17550.070.503631
AAGGGTA15600.069.5883264
TATAGGG20600.069.579552
AGTAGGG42700.068.8962252
AGAGGGC34500.068.6531453
AGGGATG31900.068.5032655
ATAGGGA24150.068.108283
ATAGCGG5600.068.050261
AAGAGGG83900.067.99952
TAGCGGG14100.067.659052
TAAGGGA18900.067.383953
TAGAGGG47950.066.743272
CGTAGGG6700.066.634292
Produced by FastQC (version 0.11.5)