FastQCFastQC Report
Mon 27 Feb 2023
SRR3126688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578060
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168660.6542128577302312No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT62490.24239156575099108No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45510.17652808701116343No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG29060.11272041767840933No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28480.11047066398764963No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26650.10337230320473534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63150.085.01251
ACGGGAT6550.075.5699544
TACGGGT1750.072.732233
AGGGCAT22100.072.509785
ATAGCGG6000.072.3353351
AGGGTAT13550.072.137085
AGTACGG3050.071.1495061
CGAAGGG21950.071.089112
AAGGGTA17450.071.0495764
ATAGGGC15500.070.5598143
TAGAGGG43100.070.549192
AGTAAGG16050.070.542381
GGTAAGG15400.070.150161
AGAGGGC32400.069.9844063
CGTAGGG6150.069.755092
AGGGTAC10050.069.2038355
CGAGGGA12400.068.811033
AAGGGAC21350.068.6694644
GAATAGG16850.068.313081
AAGGGCA37100.067.979984
Produced by FastQC (version 0.11.5)