FastQCFastQC Report
Mon 27 Feb 2023
SRR3126687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588075
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162950.629618538875419No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59340.22928238169295712No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45460.17565178752547742No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG29480.11390705447098712No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27230.10521333423490432No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25910.10011301836306907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64750.083.3357241
CGTAGGG5700.076.71062
AGTACGG3200.076.447091
AGGGCAT22750.074.9887855
ATAGCGG6800.074.717521
CGAAGGG24800.073.936732
AGGGTAC10850.073.2028665
ACGGGAT6900.072.879564
GGTAAGG15100.071.656981
ATAGGGC15400.071.411183
TAGACGG4250.070.843281
TAGGGCA14200.070.495654
AGTAGGG40550.070.147442
GTAGGGC11550.069.173223
TAAGGGA23350.069.036433
GTAAGGG41400.068.593652
AGGGCAC17000.068.560485
AGGGATG30150.067.962645
AAGGGTA17100.067.8847054
AGGGTAG10900.067.693075
Produced by FastQC (version 0.11.5)