FastQCFastQC Report
Mon 27 Feb 2023
SRR3126686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760617
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172200.6237735984383201No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61520.22284873272895153No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45910.1663034024640144No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG32270.11689415808132748No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27810.10073834943420258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67100.082.766721
ATAGCGG7600.079.2670441
ACGGGAT6450.075.0515754
CGTAGGG6250.074.4426352
AGGGTAC12100.073.022185
AGGGCAT22050.072.042775
CGAAGGG25200.070.8679052
GTAAGGG44400.070.283222
TAGCGGG18250.070.0442662
TAAGGGA24000.069.711643
AAGGGTA18900.069.627134
GTAGGGT9800.069.53583
AGTAAGG19300.069.255991
ATAGGGC16050.068.5184253
TAGGGCA12650.067.9896554
GGTAAGG17450.067.428091
TAGGGTA7350.067.140454
TGTAGGG23900.067.053742
GAGGGTA14800.067.004364
AGTAGGG43550.066.906782
Produced by FastQC (version 0.11.5)