FastQCFastQC Report
Mon 27 Feb 2023
SRR3126685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790969
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321001.1501381778156619No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57600.20637993471084773No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39590.14185037526393163No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG32370.11598122372552329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119550.087.3553851
CGTAGGG7100.073.730832
AGGGATG39850.072.994125
ATAGCGG6300.072.658941
ATAGGGC18200.070.9983143
AGTAGGG46550.069.905912
ACGGGAT7850.069.687874
GGTAAGG15700.069.433681
GAATAGG18750.068.7098851
AGGGCAT23900.068.620385
GAGGGAT30750.068.553764
TAGAGGG49950.068.452132
GTACGGG6350.068.327982
AGAGGGC37250.068.238793
TAAGGGA23100.068.187283
ATAGGGA27450.067.689723
AATAGGG43500.067.543452
AGTAAGG18100.067.5272451
ATAGAGG18550.067.161111
AAGGGCG12850.067.160954
Produced by FastQC (version 0.11.5)