FastQCFastQC Report
Mon 27 Feb 2023
SRR3126684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790832
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306141.0969488668612084No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58010.20785916171234958No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39070.13999409495089635No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG31620.11329954651516107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109750.085.916171
AGGGATG41250.072.347825
ATAGCGG5650.071.619571
TAGGGCG5850.070.697244
TAGGGCA12600.070.12344
GCATAGG7150.069.7561
ACGGGAT7750.069.738374
ATAGGGC16700.069.2299963
AGTAGGG46100.069.054052
AGGGCAT22900.068.751955
CGAAGGG26700.068.3316352
GGTAAGG18200.068.251821
AAGGGTA17100.068.160264
ATAGGGA27200.067.904623
GAATAGG18600.067.542951
AGTAAGG17900.067.292811
AGGGTAC11900.066.744495
AGAGGGC40300.066.5896453
GTAAGGG46200.066.4618762
TAACGGG9900.066.0208362
Produced by FastQC (version 0.11.5)