FastQCFastQC Report
Mon 27 Feb 2023
SRR3126682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539735
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200251.3005484710031272No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33650.21854410012112474No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22000.14288172964828363No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG16570.10761592092145726No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT16170.10501807129148846No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT15890.1031995765505103No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG15430.1002120494760462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68900.087.676091
ACGGGAT4350.075.893024
ACGGGTA750.075.459354
ATAGCGG2950.075.191061
AGGGATG21500.074.531655
GAATAGG9300.073.0751951
TAGGGCG2700.071.616514
AGTAAGG9950.070.19871
TAGAGGG25600.070.192752
ATAGGGC9650.069.887873
AGAGGGC20350.069.758183
AAGGGTA9150.069.583414
TAAGGGA13300.068.792823
TAGCGGG8400.068.499562
GGTAAGG8750.068.499291
CGTAGGG4000.068.387262
ATAGAGG10400.068.068841
GAGGGAT18750.067.4103554
AGTAGGG24500.067.184112
AGGGAAT18050.066.648075
Produced by FastQC (version 0.11.5)