FastQCFastQC Report
Mon 27 Feb 2023
SRR3126681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542113
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193721.256198475727784No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32580.2112685646252901No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20680.13410171628149167No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT16180.10492097531114776No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG16110.1044670526738313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68700.087.475311
ATAGCGG3450.080.479311
GGTAAGG7950.077.54531
AGGGATG22050.072.252125
CGAAGGG15150.071.078122
CGTAGGG4200.070.534942
GCATAGG4150.070.306411
GTAGGGC7700.068.967933
AGTAAGG8800.068.450811
GAATAGG8850.068.064091
ACGGGAT4150.067.945814
AGGGCAT13800.067.769385
ATAGGGC9900.067.408193
AGGGCAC11000.067.07595
ATAGGGA14800.067.000843
AGAGGGC22550.066.481933
AGGGACT23100.066.323155
TAAGGGA13000.066.155693
TAGAGGG29100.066.0911562
AGTAGGG24350.065.851922
Produced by FastQC (version 0.11.5)