FastQCFastQC Report
Mon 27 Feb 2023
SRR3126680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627013
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199511.2262348241839494No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32240.19815453226249574No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22400.13767560554218067No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG17130.10528496084542655No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT17070.10491618690201No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG17050.10479326225420449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.087.558591
CGTAGGG3750.076.438582
TAGGGTC3050.073.952954
AGTAGGG24450.073.032942
GGTAAGG9650.072.658661
TAGGGCA8250.072.337564
ATAGGGC9950.072.2574163
GCGTAGG2100.071.70661
GTACGGG4150.071.335642
AGGGATG23750.070.834835
CGAAGGG15450.069.954142
AGGGCAT14450.069.919465
TAAGGGA13400.069.785053
ATAGCGG3400.069.202151
ATAGGGA15250.069.022753
GTAGGGC6400.069.017943
GAGGGAT19300.068.90384
AGTAAGG11250.068.599311
TACAGGG8850.068.495262
ACGGGAT5250.068.024974
Produced by FastQC (version 0.11.5)