FastQCFastQC Report
Mon 27 Feb 2023
SRR3126679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375988
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57100.41497454919664994No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52950.38481440245118415No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39010.2835053794073786No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT33500.24346142553568784No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29350.213301278790222No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG26760.19447844021895538Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG23710.17231254923734798RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC23550.1711497483989686RNA PCR Primer, Index 41 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG6400.086.9030463
CGTTTTT34800.084.6914441
CGTAGGG4250.084.289492
TCCGCTA6900.082.010771
ACGGGTA1100.081.413024
GGTAAGG8500.080.9976961
ACGGGTC1050.080.8008964
AATGCGG3100.080.621881
CGAAGGG10250.080.4753652
CGACCAA6550.080.355138
AGGGATG22200.079.380755
GGGCGAT6900.079.00346
ATAGCGG3050.078.851341
TAGGGCG2700.078.556424
GCTACGA7050.078.2221454
TAGCGGG6700.078.090272
AAGCGGG16400.077.888662
ACGGGAT4550.077.693164
AGGACGG2500.077.336161
AGGGTAT8400.077.203455
Produced by FastQC (version 0.11.5)