FastQCFastQC Report
Mon 27 Feb 2023
SRR3126677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454275
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.40686940227948637No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA54200.3726942978460057No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39750.2733320726822644No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT33810.2324869780474807No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG28980.19927455261212632Illumina PCR Primer Index 11 (95% over 24bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28830.19824311082841964No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC27650.19012910212992729RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG25970.1785769541524127RNA PCR Primer, Index 41 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC409.094947E-1293.992927
CGAAGGG11050.087.1882
CGTAGGG4150.086.06582
ACGGGTC950.084.098934
CGTTTTT35700.083.163791
AGGGTAT8400.082.24385
AAGGGTA8650.081.496754
CGGGATA2500.080.833915
TCCGCTA8700.080.582391
ACGGGTA1400.080.565364
CGCTACG8700.079.9480063
ACGAAGG3450.079.1010061
GTAGCGG2800.078.979241
AAGCGGG17950.078.8074342
CGGGTCA1200.078.327435
GCGATAC301.4569887E-678.327438
GGGCGAT6550.078.207856
GCGAAGG6050.077.771011
AGGGATG21700.077.74995
GCTACGA8850.077.530884
Produced by FastQC (version 0.11.5)