FastQCFastQC Report
Mon 27 Feb 2023
SRR3126676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010834
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.5942617680054292No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47110.46605080557242834No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG32270.319241339329702No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG28660.28352825488655903No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC28350.28046148032218937No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22040.21803777870550456No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21930.21694956837621213No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT18040.17846649400396108No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGT13170.1302884548798319No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA12500.12366026469232339No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10570.10456711982382864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG1200.094.300751
CGTTTTT28550.086.5387041
TAACGGG2750.083.980062
CGAAGGG7500.082.323312
GGTAAGG5050.082.163041
GACCGAT1050.080.561338
CTATAGG650.079.7929461
CGAGGGC2600.079.749413
AGGGCAT7150.079.52855
AGGGTAC3200.079.302565
TAGGGCG1250.079.169414
CGTAGGG3700.078.977392
TAGCGGG4900.078.87342
GATTAGG3000.078.583971
AGAGGGC8900.078.364593
GGGTACG301.4570978E-678.3235246
CGGGTAT850.077.402075
ACGAGGG9400.077.215712
GCAAGGG13500.076.458042
GTAAAGG6850.075.7159351