FastQCFastQC Report
Mon 27 Feb 2023
SRR3126675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015566
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56850.559786365435629No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45050.4435950002264747No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG33160.32651742968945396No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC30720.3024914185784085No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG29440.2898876094709748No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22190.21849884694840116No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21870.21534789467154278No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTATCGT19800.19496517213061484No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT17400.1713330300541767No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA12880.12682582914355148No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGT12110.1192438502273609No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10810.10644310660262357No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10210.10053507108351402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG950.089.185961
GGTAAGG4950.086.53341
CGTTTTT29150.083.483291
ACGGGAT1700.082.934494
AGGGCAT7950.082.760625
GTGTAGG3150.082.1863561
CGAGGGT1850.081.290743
AGAGGGC8500.080.173
TAACGGG2750.078.673812
TAGCGGG4250.078.573212
ATAGCGG2100.078.4506151
GGGTACG600.078.327026
CGAAGGG8500.078.0198752
CGGGTAA1150.077.6459055
GCATAGG2550.077.5276641
TCGAAGG1400.077.3298951
GACCGAT1100.076.9028858
GAATAGG7000.076.6574551
TCACGGG1350.076.646792
CGTAGGG2900.076.226322