FastQCFastQC Report
Mon 27 Feb 2023
SRR3126674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054030
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61150.5801542650588692No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46580.44192290542014934No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG34670.3289280191265903No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC31200.29600675502594803No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG30840.2925912924679563No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22700.21536388907336604No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21230.20141741696156656No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT18410.17466296025729816No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTATCGT14560.13813648567877573No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGT13770.13064144284318283No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA13640.12940808136390805No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10810.10255875069969544No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10580.10037664962097854No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC156.90402E-493.993267
AGGGCAT8350.084.987925
TAACGGG3100.084.897132
GGTAAGG5800.083.542761
AGTACGG1300.083.23081
CGTTTTT28400.082.98871
AGGGTAC2500.082.714085
CGAAGGG8300.082.1025542
CGAGGGT1150.081.733273
GACCGAT650.079.532768
GATTAGG2450.078.725851
ATAGGGC4750.078.162823
CGTAGGG2950.078.06222
ACGGGAT1750.077.8801354
ATAGCGG2000.077.6217651
GTAGAGG6050.076.980261
ACGAGGG8650.076.607232
ACGGGTC1350.076.5871054
GATAAGG7100.074.872041
AGACGGG7600.074.8235862
Produced by FastQC (version 0.11.5)